Lack of an inhibitory influence of oocytes upon luteinization of porcine granulosa cells in culture
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چکیده
منابع مشابه
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Hormone-stimulated secretion of luteinization factor in porcine granulosa cells.
Luteinization stimulator (LS) is an intrafollicular compound which was shown to be released by granulosa cells under in vitro conditions with stimulatory effects on immature granulosa cell differentiation. This study was undertaken to determine the effects of various endocrine agents which are involved in the regulation of ovarian function on LS secretion by porcine granulosa cells isolated fro...
متن کاملLuteinization factor-stimulated steroidogenesis in porcine granulosa cells.
Luteinization stimulator (LS), an intrafollicular compound of preovulatory (5-8 mm) follicles, increased both the basal and gonadotropins-stimulated production of progesterone by immature (1-3 mm) granulosa cells. The mechanism by which LS enhance steroidogenesis was investigated by studying the modulation of progesterone biosynthesis from exogenous cholesterol and pregnenolone in cultured porc...
متن کامل[An inhibitory influence of porcine vasoactive intestinal peptide upon mouse oocyte meiosis in culture].
The germinal vesicle (GV) of follicle-enclosed oocytes in mice remains arrested at the dictyate state of meiosis. Upon releasing the oocytes from the follicles, the meiotic process resumes, leading to dissolution of the GV, suggesting that factors in the follicular constituents sustain the meiotic arrest of oocytes. Vasoactive intestinal peptide (VIP) was demonstrated in the ovary and found to ...
متن کاملThe Effect of Granulosa Cells Co-culture and Retinoic Acid on Maturation and Development of Immature Mouse Oocytes in Vitro
Purpose: The purpose of this study was to develop an appropriate medium for in vitro maturation (IVM) of immature mouse oocytes. Materials and Methods: Germinal vesicle of female NMRI mouse oocytes (6-8 weeks old) were collected from ovaries and cultured in maturation medium MEM-a, supplemented with: 100 mlIU/ml rFSH+7.5 IU/ml hCG+5% FCS (Control group) and 2mM all- trans retinoic acid (t-RA) ...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1977
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0500103